Phospho-stat3 tyr705 cellular kit

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August undefined, 2024

WebThe Cell-Based Stat3 (Tyr705) ELISA kit is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in ... Anti-Phospho-Stat3 (Tyr705) Anti-Stat3 EGF concentrations 0 20 100 ng/ml Fig. 3A. A431 cells were stimulated by different concentrations of EGF for 10 min at 37 o C m 0. ... WebTumor heterogeneity dominates tumor biological behavior and shapes the tumor microenvironment. However, the mechanisms of tumor genetic features modul…

MILLIPLEX MAP Phospho/Total STAT3 Magnetic Bead 2-Plex Kit - Cell …

WebApr 23, 2012 · In the STAT3 (Tyr705) In-Cell ELISA Kit, cells are seeded into a 96 well tissue culture plate. The cells are fixed after various treatments, inhibitors or activators. After … WebHere, we investigated the potential effects of IL-32α on epithelial–mesenchymal transition, metastasis, and invasion, and the JAK2/STAT3 signaling pathway in pancreatic cancer … phil heselich

, Stat3-Tyr705磷酸化位点抗体#9131R-毕得医药

WebSTAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but ... WebThe Total STAT3 cellular assay kit is designed to monitor the expression level of STAT3, whether phosphorylated or unphosphorylated. It is compatible with our Phospho-STAT3 kit, and enables the analysis of phosphorylated and total proteins from a single sample for better readout of the JAK/STAT signaling pathway. phil hesketh trust mediation

STAT3 (Phospho-Tyr705) Fluorometric Cell-Based ELISA Kit

STAT3 (Phospho) [pY705] Human InstantOne™ ELISA Kit

WebPhospho-Stat3 (Tyr705) Antibody detects endogenous levels of Stat3 only when phosphorylated at Tyr705. The antibody does not cross-react with other Stat proteins … WebStat3-Tyr705磷酸化位点抗体#9131R, 是JAK/STAT Signaling类化合 ... Anti-infection Apoptosis Autophagy Cell Cycle Cytoskeleton DNA Damage Endocrinology/Hormones … phil heskethWebThe Phospho-STAT3 (Tyr705) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. The first antibody is selected for its specific … phil hesketh mediator

"WebThe kit can be used for measuring the relative amounts of phosphorylated STAT3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or … " - Phospho-stat3 tyr705 cellular kit

Phospho-stat3 tyr705 cellular kit

STAT3 (Tyr705) In-Cell ELISA Kit (ab126427) Abcam

WebMouse/Human/Rat Phospho-STAT3 (Tyr705) (Cell-Based Phosphorylation ELISA) ELISA Kit - LS-F1625. LS-F1625 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human STAT3 in samples of Adherent Cell Cultures. It is based upon a Cell-Based Phosphorylation assay principle. WebOncostatin M (OSM) and leukemia inhibitory factor (LIF) signaling protects the heart after myocardial infarction (MI). In mice, oncostatin M receptor (OSMR) and leukemia inhibitory factor receptor (LIFR) are selectively activated by the respective cognate ligands while OSM activates both the OSMR and LIFR in humans, which prevents efficient translation of …

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WebThe MILLIPLEX® MAP STAT Phosphoprotein 5-plex Magnetic Bead Kit is used to detect changes in phosphorylated STAT1 (Tyr701), STAT2 (Tyr690), STAT3 (Tyr705), STAT5A/B (Tyr694/Tyr699), and STAT6 (Tyr641) in cell lysates using the Luminex®® system. The MILLIPLEX® MAP assay is a rapid, convenient alternative to Western WebDec 1, 2024 · E STAT3 mutants were overexpressed in HM20 and CL1-5 STAT3-KO [an sgRNA was designed to target human STAT3, and STAT3 knockout was performed by CRISPR/Cas9 technology] cells prior to incubation ...

WebMembranes were probed with 0.5 µg/mL (1:1000 dilution) of purified anti-STAT3 Phospho (Tyr705) antibody (clone 13A3-1) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Purified anti-STAT3 antibody (clone 4G4B45) was used as a ... WebApr 12, 2024 · (A) The time dependence of FLU effect on STAT3 and phospho-STAT3 (Tyr705) expression. Cell were treated with 0.5 µM FLU and harvested in three time …

WebIGFBP2 knockdown reduces STAT3 activating phosphorylation Representative western blot images STAT3 phosphorylation in IGFBP2 knockdown stable cell lines a UW228 (n = 3) b ONS76 (n = 5) c PZp53Med (n = 3) and d NeuroD2: Smo/A1 mouse primary MB cells (n = 2). WebDec 1, 2024 · Cell Signaling Technology's Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb is a Rabbit monoclonal antibody. The Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb Antibody was generated using STAT3 as the antigen. It reacts with Human, Mouse, Rat, and Non-Human Primate. This antibody has been shown to work in applications such as: …

WebIt can be used for measuring the relative amount of Stat1 (pTyr 701), Stat3 (pTyr 705) and Stat5 (pTyr 694) phosphorylation and screening the effect of various treatment, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines (Stat5 only can be used for detecting human cell lines). 免疫原

WebPhospho-STAT3 (Tyr705) Colorimetric Cell-Based ELISA Kit (OKAG02059) Aviva Systems Biology Aviva Systems Biology Email Us Catalog No: OKAG02059 Size: Two 96-Well … phil hess wellspanWebSTAT3 (Phospho-Tyr705) Colorimetric Cell-Based ELISA Kit SKU: CBCAB00020 Product type: ELISA Kit ELISA Type: Cell Based Research Area: Immunology €599 Quantity: … phil heseltineWebphosphorylated at Tyr705. Upon activation, STAT3 is phosphorylated and after lysis of the cell membrane, phospho-STAT3 (Tyr705) can be detected using the kit reagents. FRET signal. Phospho-STAT3 Phospho-STAT3. Phospho-STAT3 Eu . Cryptate antibody. Phospho-STAT3 d2 antibody. Figure 1: Principle of HTRF sandwich assay. As shown … phil hess photographyWebStore at 4°C PathScan® Phospho-Stat3 (Tyr705) Sandwich ELISA Kit n 1 Kit (96 assays) ... PathScan® Sandwich ELISA Kit) in purified water. 8. Cell Lysis Buffer: 10X Cell Lysis Buffer #9803 or 1X Cell Lysis Buffer #7018: This buffer can be stored at 4°C for short-term use (1–2 weeks). Recommended: phil hess philhavenWebWestern blot analysis of Phospho-STAT3 pTyr705 in whole cell extracts of HeLa cells treated with 100 ng/mL insulin for 15 min (lane1), without insulin treatment (lane 2), and … phil hesseWebSTAT3 is activated by Tyr705 phosphorylation in response to membrane factors such as interleukin-6 (IL-6). 45 It has also been shown that Ser727 of STAT3 is phosphorylated by various kinds of kinases in various cancers. 46–49 However, some reports have shown that the Ser727 site phosphorylation suppresses STAT3 activity and cell proliferation ... phil hess obituaryWebMouse/Human/Rat Phospho-STAT3 (Tyr705) (Cell-Based Phosphorylation ELISA) ELISA Kit - LS-F1625. Submit Review LSBio Guarantee. Description: LS-F1625 is a 96-well enzyme … phil hessler