Goldenstar rt6 cdna synthesis mix
WebAug 16, 2024 · The reverse transcription was carried out with Goldenstar RT6 cDNA Synthesis Kit (TSK302M, Tsingke, Beijing, China). An RNase-free EP tube was prepared, and gDNA remover, 10× gDNA remover Buffer, dNTP Mix, Oligo dT, Randomer, DTT, and RT6 were added with 1 μ L, respectively. WebSep 6, 2024 · The first strand of cDNA was synthesized according to the instructions for the Goldenstar TM RT6 cDNA synthesis Mix (TIANGEN, Beijing, China). Subsequently, qRT-PCR was performed using the SYBR PrimeScript RT-PCR Kit (TIANGEN, Beijing, China) with SYBR Green dye. The maize actin gene ZmActin was used as the internal control.
Goldenstar rt6 cdna synthesis mix
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WebIf you are new to cDNA synthesis or experience researcher wanting to optimize your protocol, consider these five critical steps to help you ensure your cDNA synthesis results in highest efficiency. Learn reverse transcription basics Find reverse transcriptases See also five steps for fast RT-PCR. Step 1 Prepare. sample. Step 2 Remove. genomic DNA. Web10 Pomeranian Mix Dogs You Never Heard Of - Part 2. All Web Stories Premier Pups > Locations > Fawn Creek, KS > Pomeranian Contact Info Puppy Agents: 740-809-3074 …
WebSep 10, 2024 · Using cDNA originating from CB03234-S as a template, any intergenic region embodied in transcription unit would give a clear PCR product, whereas those failing to generate PCR fragments could constitute promoter areas delimiting different transcription units. ... China), and reverse transcribed into cDNA by using Goldenstar RT6 cDNA … WebFeb 23, 2024 · Genomic DNA was removed according to Goldenstar TM RT6 cDNA synthesis kit (Sangon Biotech Co. Ltd at Shanghai). For reverse transcription, 2 μg of total RNA was used to generate cDNA through the …
WebTotal RNA was extracted from the cells using Trizol reagent (Ambion, Life technologies), and 1 μg of RNA was reverse-transcribed using the Goldenstar RT6 cDNA Synthesis Mix (Takara, Kyoto, Japan) according to the manufacturer’s instructions. WebDec 1, 2024 · The Goldenstar™RT6 cDNA Synthesis Kit (TsingKe, Beijing, China) was used for the cDNA synthesis in a 10 μL reverse-transcription reaction mixture (5× …
WebApr 1, 2024 · Goldenstar™ MIX (TSINGKE) were used for amplifying cDNA under the following conditions: 98 °C for 2 min followed by 35 cycles of 98 °C for 10 s, 58 °C for 10 …
Web丁香通为您找到150条一次Pcr扩增有多少基因厂家的工商注册年份、员工人数、年营业额、信用记录、主营产品、相关一次Pcr扩增有多少基因产品的供求信息、交易记录等企业详情。丁香通为买家提供用户服务，诚信保障等服务，批发采购一次Pcr扩增有多少基因，上丁香通。 home office family and human rightsWebDec 1, 2024 · The reverse transcription experiment was carried out by Goldenstar™ RT6 cDNA Synthesis Kit Ver2 (Tsingke, Beijing, China). The cDNA product was diluted as a qPCR template and amplified with 2×T5 Fast qPCR Mix(SYBR Green I, Tsingke, Beijing, China). A Real-time PCR System (BIOER) was used to amplify. hinge folding mechanism bikeWebMar 5, 2024 · The Goldenstar RT6 cDNA Synthesis Kit Ver.2 (TsingKe, China) was used following the manufacturer’s instructions. ... (Bio-Rad, USA), with a total volume of 20 μL reaction system containing 10 μL of 2 × T5 Fast qPCR Mix (SYBR Green I) (TsingKe, China), 0.8 μL of 10 μM Primer F, ... home office fbis programmeWebApr 26, 2024 · And there were higher expression levels of lignin synthesis- and lignifications-related genes in the GhWAT123-silenced plants. ... following the manufacturer’s protocol. First-strand cDNA was synthesised from 3 μg of total RNA using the Goldenstar RT6 cDNA Synthesis kit Ver. 2 (Tsingke, Beijing, China) to analyse the … home office fast track visaWeb产品中的Goldenstar TM RT6逆转录酶由改造的莫洛尼氏鼠白血病病毒逆转录酶(M-MLV RT)经体外大肠杆菌重组表达而来。野生型M-MLV RT在分子生物实验中有两个明显的缺 … hinge folding monitor armWebOct 18, 2024 · Total RNA from the four samples was extracted with an RNA extraction kit (TSINGKE, Beijing, China). Subsequently, cDNA was synthesized using the Goldenstar RT6 cDNA Synthesis Kit Ver.2 kit (Beijing TsingKe Biotech Co., Ltd.). qRT-PCR was performed using the CFX96TM real-time system (Bio-Rad, California, USA) and 2 × … home office family consent formWebGoldenstar® RT6. cDNA Synthesis Mix (gDNA remover and Rnasin selected)/50℃反应，使用方便，反应快速，热稳定性强，含DNase和Rnase抑制剂/反转录系列试剂/擎科生 … home office family reunion contact