WebJul 10, 2024 · This protocol provides an overview for real time measurement of immune cell killing of adherent or non-adherent tumor cells. The highly flexible co-culture assay format is amenable to both... WebWe optimized IncuCyte® CytoLight Rapid Green Reagent for live cell cytoplasmic labeling with Jurkat cells, pmel-1 T cells, and IncuCyte® CytoLight Rapid Red Reagent with B16F10 melanoma cells. The optimal concentrations (based on minimal decrease in cell viability and highest percentage of stained cells) were: Jurkat: 1 µM (green)
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WebJan 1, 2024 · CD19+ MEC-1 cells were stained with 0,75 µM Incucyte Cytolight Rapid Dye Green (Sartorius, Göttingen, Germany; no. 4705). In total 35 × 10 3 MEC-1 were cocultured with patient-derived CAR T cells in a 96-well plate at a 1:1 ratio. CD19-U937 cells were used as a negative control. Healthy donor (HD) CAR T cells served as a control and were ... WebFeb 20, 2024 · The technique of live-cell analysis involves the real-time quantification of living cells' behavior using time-lapse imaging, spanning from hours to weeks. This method comprises: Acquisition of... easy games to play with kids indoors
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WebJun 1, 2024 · Once the iBEC barrier was formed, CAR-F263 cells were labeled with 0.33 µM Incucyte ® Cytolight Rapid Green Dye (Sartorius) for 30 min and infused 0.5 µl/min for 10 min for CAR-F263 T cell accumulation followed by 0.1 µl/min for 24 h for constant flow condition. Phase contrast images and fluorescent images (red channel, ex., 565–605 nm ... WebDescription: REAGENT CYTOLIGHT RAPID RED 1MM 20UL PK5 Retrieving Pack KIT IQUE FLUIDICS MAINTENANCE SCREENER Catalog Number: (MSPP-91095) Supplier: SARTORIUS CORPORATION MS Description: KIT IQUE FLUIDICS MAINTENANCE SCREENER Retrieving Each 1 - 16 of 55 1 2 3 4 Next Recommended for You WebDec 1, 2024 · Briefly, MJ cells endogenously expressing ICOS were first labeled with the Incucyte CytoLight Rapid Green Reagent (Sartorius) to enable detection of the cytoplasm. Serial dilutions of either KY1044, a CD71 antibody (positive control; Sigma, catalog no. SAB4700520-100UG) or a human IgG1 isotype control (negative control) were … easygame官网